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Pharma & Biotech

Cell Line NucleofectorTM Kit V

Catalog #: VVCA-1003
For efficient transfection of cell lines, e.g. HepG2, HL-60, Jurkat, K-562 , MCF7, SH-SY5Y, or THP-1, in the NucleofectorTM I/II/2b Device
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Product Overview

The Cell Line NucleofectorTM Kit V is one of our five NucleofectorTM Kits suited for transfecting cell lines in the NucleofectorTM 2b Device.

This kit has been determined to be optimal for transfecting following cell types:

32D
3T3-L1 pre-ad
A20
A375
A7r5
AGS
ARPE-19
B16-F10
BA/F3 (DSMZ)
C2C12
C6
Capan-1
CHO [suspension]
CHO-S [suspension] (Life Technologies)
COS-1
HaCaT
HCT116
HEK293
HepG2
HL-60
HUV-EC-C
Jurkat
K-562 (DSMZ)
MCF7
MDA-MB-231
MDA-MB-468
NB-4 (DSMZ)
NCTC clone 929
Neuro-2a [N2a]
NG108-15
P19
PC-12
PC-3
Raji
Ramos
RAW 264.7
RBL-1
S49
Saos-2
Schneider‘s Drosophila Line 2
SH-SY5Y
SK-N-SH
SK-OV-3
SW480
T/C-28 a2
T/G HA-VSMC
T-47D
THP-1
U-2 OS
Vero

The respective cell type-specific Optimized Protocols are available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.

Benefits

  • High transfection efficiencies in difficult-to-transfect cell lines

Applications

  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells 
  • Low-throughput transfection in single cuvette format

Content & Storage

Content

4 x 2.25 mL NucleofectorTM Solution V
4 x 0.5 mL Supplement 1
4 x 30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
4 x 25 Aluminum cuvette (100 µL)
4 x 25 Single use pipettes

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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Need technical data?

Browse our Cell and Transfection, Citation, FAQ and Culture Media Technical Databases.

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