text.skipToContent text.skipToNavigation
Need help? Please contact us
Pharma & Biotech

Cell Line Nucleofector™ Kit T

Catalog #: VVCA-1002

For efficient transfection of cell lines, e.g. A549, Caco-2, CHO-K1, HT-, or U-87 MG, in the NucleofectorTM I/II/2b Device

This product will be discontinued December 2022. Please select the 25 rct kit instead.


Product Overview

The Cell Line NucleofectorTM Kit T is one of our five NucleofectorTM Kits suited for transfecting cell lines in the NucleofectorTM 2b Device. This kit has been determined to be optimal for transfecting following cell types:

  • A431
  • A549
  • Caco-2
  • CHO-K1
  • D1
  • HT-1080
  • NALM-6 (DSMZ)
  • NRK
  • P815
  • RBL-2H3
  • T84
  • TF-1 U
  • -87 MG

The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.


  • High transfection efficiencies in difficult-to-transfect cell lines


  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells 
  • Low-throughput transfection in single cuvette format

Content & Storage


4 x 2.25 mL NucleofectorTM Solution T
4 x 0.5 mL Supplement 1
4 x 30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
4 x 25 Aluminum cuvette (100 µL)
4 x 25 Single use pipettes


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Subscribe to our eNewsletter

Keep up to speed on the latest scientific developments, events, tips and tools from Lonza.

Interested in our Webinars?

Need a break from your daily routine? Grab a coffee and check out our free webinars. 


Knowledge Center

Need technical data?

Browse our Cell and Transfection, Citation, FAQ and Culture Media Technical Databases.