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Pharma & Biotech

Basic Parasite Starter NucleofectorTM Kit

Catalog #: VMI-1001
For determination which of the two NucleofectorTM Kits is best suited for efficient transfection of a specific parasite type in NucleofectorTM I/II/2b Device
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Product Overview

The Basic Parasite NucleofectorTM Starter Kit is used to determine which one of our two NucleofectorTM Kits is best suited for transfecting a specific parasite type in NucleofectorTM 2b Device. Parasitic protozoa infect vertebrates and invertebrates and some are even parasitic in plants. In humans, they can cause severe diseases, such as Malaria (Plasmodium), Sleeping Sickness (Trypanosoma) or Leishmaniasis (Leishmania). Nucleofection has proven to provide considerably higher transfection efficiencies (e.g., in Plasmodium berghei and Trypanosoma brucei) compared to standard methods, such as electroporation or particle bombardment. Due to significant genotypic and phenotypic diversity between species and life cycles, we have developed two Basic Parasite NucleofectorTM Kits (1 and 2) and an easy-to-use Basic Parasite NucleofectorTM Starter Kit to determine which of the 2 kits is optimal for a specific parasite type.

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Benefits

  • Increased transfection efficiencies compared to standard methods, such as electroporation or particle bombardment

Applications

  • Determination of optimal NucleofectorTM Kit for specific parasite type

Content & Storage

Content

0.45 mL NucleofectorTM Basic Solution 1 for Parasites
0.45 mL NucleofectorTM Basic Solution 2 for Parasites
0.1 mL Supplement 1
0.1 mL Supplement 3
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
10 Aluminum cuvette (100 µL)
10 Single use pipettes

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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