Basic Parasite Starter NucleofectorTM Kit
What You May Also Need
Product Overview
The Basic Parasite NucleofectorTM Starter Kit is used to determine which one of our two NucleofectorTM Kits is best suited for transfecting a specific parasite type in NucleofectorTM 2b Device. Parasitic protozoa infect vertebrates and invertebrates and some are even parasitic in plants. In humans, they can cause severe diseases, such as Malaria (Plasmodium), Sleeping Sickness (Trypanosoma) or Leishmaniasis (Leishmania). Nucleofection has proven to provide considerably higher transfection efficiencies (e.g., in Plasmodium berghei and Trypanosoma brucei) compared to standard methods, such as electroporation or particle bombardment. Due to significant genotypic and phenotypic diversity between species and life cycles, we have developed two Basic Parasite NucleofectorTM Kits (1 and 2) and an easy-to-use Basic Parasite NucleofectorTM Starter Kit to determine which of the 2 kits is optimal for a specific parasite type.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Benefits
- Increased transfection efficiencies compared to standard methods, such as electroporation or particle bombardment
Applications
- Determination of optimal NucleofectorTM Kit for specific parasite type
Content & Storage
Content
0.45 mL NucleofectorTM Basic Solution 1 for Parasites
0.45 mL NucleofectorTM Basic Solution 2 for Parasites
0.1 mL Supplement 1
0.1 mL Supplement 3
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
10 Aluminum cuvette (100 µL)
10 Single use pipettes
