Trypsin/EDTA (1X) Solution
Trypsin/EDTA (1X), includes 170,000 U/L trypsin 1:250 and 0.2g/L Versene® (EDTA) manufactured with irradiated porcine trypsine, tested for porcine parvovirus and mycoplasma, 100 mL
Trypsin-Versene® (17-161) or Trypsin-EDTA (ethylenediaminetetraacetic acid) is a combined reagent used to remove adherent cells from the culture vessel surface into suspension.
The EDTA, a chelator of divalent cations, is added to trypsin solutions to enhance activity.
The calcium and magnesium in the extracellular matrix, which aids in cell-cell adhesion, also obscures the peptide bonds that trypsin acts on.
The EDTA is added to remove the calcium and magnesium from the cell surface which allows trypsin to hydrolyze specific peptide bonds.
The trypsin activity can be halted by adding a serum media mixture or a non-animal trypsin inhibitor (from soybean for example) in serum-free media systems.
BE17-161E is supplied at a ready-to-use dilution. BE17-161E contains 170 000 U/L porcine trypsin 1:250 and 0.2 g/L Versene (EDTA).
Trypsin rawmaterial is tested for absence of porcine parvovirus and mycoplasma. Product is manufactured with irradiated trypsin.
Content & Storage
100 ml bottle