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Pharma & Biotech

OsteoLyseTM Assay Kit

Catalog #: PA-1500

OsteoLyseTM Assay Kit (contains Human Collagen) provides an easy to use protocol for quantitatively measuring in vitro osteoclast-mediated bone resorption in a high-throughput format.

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Product Overview

The OsteoLyseTM assay kit provides an easy to use protocol for quantitatively measuring in vitro osteoclast-mediated bone resorption in a high-throughput format. Osteoclasts can be seeded onto the OsteoLyseTM plate using traditional cell culture protocols.

The assay directly measures the release of Europium-labeled collagen fragments into the osteoclast cell culture supernatant via time resolved fluorescence, indicating their resorption activity levels.

 

Benefits

  • Convenient - Human collagen covalently bound to wells in plate eliminating the need to purchase bone matrices separately
  • Easy to use - Cells can be seeded onto the surface of the OsteoLyseTM plate as used in traditional cell culture protocols
  • Effective - Resorptive activity easily measured by simply sampling the cell culture supernatant and counted via time-resolved fluorescence

Content & Storage

Content

  • 1 x 96-well OsteoLyseTM Plate
  • 1 x Fluorophorereleasing Reagent
  • 1 x 96-well Black Assay Plate 

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

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