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Pharma & Biotech

RAFTTM 24-Well Small Transwell Kit, 3D Reagents and Transwell Absorbers for 24-Well Inserts - 12 Reactions

Catalog #: 016-1R18
RAFTTM 24-Well Small Transwell Kit, 3D Reagents and Transwell Absorbers - 12 Reactions
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Product Overview

The RAFTTM 3D Cell Culture System uses rat tail collagen type 1 along with patented absorber technology to create a realistic cellular environment to study cells' complex behavior. RAFTTM Kit is a simple-to-use 3D cell culture system that enables the production of robust, reproducible 3D cultures in less than an hour.

What does the RAFTTM System Consist of?

  • RAFTTM Reagent Kit – consists of rat tail collagen 1 as the extracellular matrix scaffold and a specially-designed neutralizing solution that enables neutralization to be carried out in a single step instead of an error-prone and tedious titration method. The kit also contains 10X MEM to support cells through the RAFTTM Process.  
  • Novel RAFTTM Absorbers – The biocompatible absorbers are designed to work with standard 96- or 24-well cell culture plates as well as 24-well cell culture inserts. Once the cells are mixed with RAFTTM Reagents, the absorbers are utilized to compress and absorb excess liquid from the collagen mixture. This results in the creation of well-controlled, high-density collagen scaffolds embedded with cell type(s) of choice. The RAFTTM System is one of the few commercially available kits that allows users to create these high-density collagen scaffolds that are representative of a natural, more in vivo like environment for cells to grow and interact more efficiently.

RAFTTM 24-well Insert Kit for Cell Culture Inserts (catalog number 016-1R25 and 016-1R33). RAFTTM Insert Absorbers are designed to be used with 24-well permeable membrane cell culture inserts. This additional kit is offered to enhance the versatility of the system further by allowing researchers to generate co-culture models, and barrier models including air-lift models. 

Content & Storage

Content

1 x Bottle Collagen Type I ≥ 25 mL
1 x Bottle 10X MEM ≥ 15 mL
1 x Bottle Neutralizing Solution ≥ 10 mL
4 x Mixing Vessels
12 x Absorbers for 24-Well Transwell Inserts

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

A Three-Dimensional RAFTTM Co-Culture as Advanced Model for Breast Cancer Drug Discovery

Presented at AACR 2017, we demonstrate here the feasibility of co-culturing human mammary fibroblasts and human mammary epithelial cells in RAFTTM 3D Culture System as a 3D breast cancer model.

Construction of a Full Thickness Skin Model Using RAFTTM 3D Cell Culture System

In this Technical Note, we describe the procedure in constructing full thickness (FT) skin models using the RAFTTM 3D Culture System with primary Human Neonatal Epidermal Keratinocytes (NHEK) and primary Human Neonatal Dermal Fibroblasts (NHDF).

Generation and Analysis of Complex Tissue Models Using the RAFTTM System

Webinar

Three experts describe how they use the RAFTTM 3D Cell Culture System for the creation of different complex tissue models.

Presented by Biotechniques

RAFTTM Information Request

Is the RAFTTM System applicable to your project?