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Pharma & Biotech

RAFT™ 24-Well Large Transwell Kit, 3D Reagents and Transwell Absorbers for 24-Well Inserts - 48 Reactions

Catalog #: 016-1R25
RAFTTM 24-Well Large Transwell Kit, 3D Reagents and Transwell Absorbers - 48 Reactions
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Product Overview

The RAFTTM 3D Cell Culture System uses rat tail collagen type 1 along with patented absorber technology to create a realistic cellular environment to study cells' complex behavior. RAFTTM Kit is a simple-to-use 3D cell culture system that enables the production of robust, reproducible 3D cultures in less than an hour.

RAFTTM 24-well Insert Kit for Cell Culture Inserts consists of enough reagents for 48 microtissues including:  

RAFTTM Reagent Kit (Cat# 016-0R94) – consists of rat tail collagen 1 as the extracellular matrix scaffold and a specially-designed neutralizing solution that enables neutralization to be carried out in a single step instead of an error-prone and tedious titration method. The kit also contains 10X MEM to support cells through the RAFTTM Process. 

RAFTTM 24-well insert Absorbers (Cat# 016-1R33) – RAFTTM Insert Absorbers are designed to be used with 24-well permeable membrane cell culture inserts. Once the cells are mixed with RAFTTM Reagents, the absorbers are utilized to compress and absorb excess liquid from the collagen mixture. This results in the creation of well-controlled, high-density collagen scaffolds embedded with cell type(s) of choice. The RAFTTM System is one of the few commercially available kits that allows users to create these high-density collagen scaffolds that are representative of a natural, more in vivo like environment for cells to grow and interact more efficiently.

Applications for RAFTTM 24-well Insert Kit include:

  • Blood brain barrier modes
  • Full thickness skin models
  • Airway models

Visit our Applications page for tutorials and publications

 

Content & Storage

Content

1 x Bottle Collagen Type I ≥ 100 mL
1 x Bottle 10X MEM ≥ 15 mL
1 x Bottle Neutralizing Solution ≥ 10 mL
4 x Mixing Vessels
48 x Absorbers for 24-Well Transwell Inserts

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

A Three-Dimensional RAFT™ Co-Culture as Advanced Model for Breast Cancer Drug Discovery

Presented at AACR 2017, we demonstrate here the feasibility of co-culturing human mammary fibroblasts and human mammary epithelial cells in RAFT™ 3D Culture System as a 3D breast cancer model.

Construction of a Full Thickness Skin Model Using RAFTTM 3D Cell Culture System

In this Technical Note, we describe the procedure in constructing full thickness (FT) skin models using the RAFTTM 3D Culture System with primary Human Neonatal Epidermal Keratinocytes (NHEK) and primary Human Neonatal Dermal Fibroblasts (NHDF).

Generation and Analysis of Complex Tissue Models Using the RAFT™ System

Webinar

Three experts describe how they use the RAFTTM 3D Cell Culture System for the creation of different complex tissue models.

Presented by Biotechniques

RAFT™ Information Request

Is the RAFTTM System applicable to your project?