Product Overview
ViaLight® Plus BioAssay Kits are designed to provide unprecedented speed and sensitivity for cytotoxicity and cell proliferation studies. The ViaLight® Assay incorporates bioluminescent detection of cellular ATP as a measure of viability. Measurement of ATP is the most accurate, effective, and direct way of determining the number of living cells in culture.
The ViaLight® Plus BioAssay is designed to deliver a high, stable luminescent signals for an extended period of time for greater experimental design flexibility. The easy two-step, non-radioactive assay is scalable for high-throughput applications in both 96-well and 384-well formats. The assay can be run on a variety of luminometers.
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Benefits
- Fast - Results from a 96 well plate can be processed and analyzed in as little as 10 minutes.
- Sensitive - Detect as few as 10 cells allowing for lower seeding densities and more assays.
- Convenient - Simply add two reagents directly to your culture well and read. No cell washing or medium removal required.
- Flexible - Dynamic range of 5 decades and excellent performance with both adherent or suspension cultures allows you to design the experiment for your needs.
- Robust - Stable bioluminescent signal (half-life >5 hours) facilitates manual or batch processing. And, unlike MTT or Alamar Blue assays, ViaLight® Bioassays are not prone to interference due to culture condition changes.
Storage and Content
10000 tests (Sufficient for 100 plates)
- 10 x LT27-213 ATP monitoring reagent plus, Lyophilized
- 10 x LT27-080 Assay buffer, 100 mL bottles
- 5 x LT27-077 Cell lysis reagent, 100 mL bottles