Rat Neonatal Dorsal Root Ganglion (DRG) Neurons
Rat Neonatal Dorsal Root Ganglion (DRG) Neurons
Cryopreserved ampule of Rat Neonatal DRG Neurons containing ≥ 200,000 cells
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Product Overview
Ready-to-use CloneticsTM Rat Dorsal Root Ganglion Neurons are suspensions of high quality sensory neurons prepared by standardized methods, and are ready for immediate culture.
Each vial of dorsal root ganglia cells contains approximately 200,000 cells in 0.25 ml suspension. Cell death will occur during the first few days after plating and debris will be observed. This is normal. After approximately 4 days in culture, the cells will form a neurite network and by the 7th day, debris will be minimal.
In the absence of mitotic inhibitors, the neurons tend to cluster (ganglionate) and detach from the substrate. A ganglion is a group of nerve cells forming a nerve center, especially one located outside the brain or spinal cord.
Dorsal root ganglion, also called spinal ganglion, is the ganglion of the posterior root of each spinal segmental nerve, containing the cell bodies of the unipolar primary sensory neurons. Dorsal root ganglion cells are pseudounipolar cells. Pseudounipolar cells have 2 axons rather than an axon and dendrite. One axon extends centrally toward the spinal cord; the other axon extends toward the skin or muscle.
Recommended Medium: PNGMTM BulletKitTM(CC-4461)
Request More InfoBenefits
- Ready-To-Use
- No tissue dissection or animal handling required
- Increased data reproducibility
- Characteristic morphology and staining tests confirm tissue origin and cell type
- Negative for mycoplasma, bacteria, yeast and fungi
- Certificate of Analysis (CoA) is available for every batch
Applications
- Neurogenesis
- Neurotransmitter Function
- Gene Expression
- Signaling Pathways
- Electrophysiology
- Neurotoxicity
- Drug/Compound Screening
- Advanced Cell Culture Models
Storage and Content
≥200,000 cryopreserved cells per ampuleSDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Instructions - Rat Neonatal DRG Neuronal Cell System
Instructions for proper use and culture of Neonatal Rat Dorsal Root Ganglion Neurons -
Supplemental Inst. - Neuronal Plate Coating Instructions
Instructions of cell culture plate coating procedure required for successful neuronal culture -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
TechSheet - Rat Neonatal DRG Neuronal Cell System
Technical tips for culturing Rat Neonatal Dorsal Root Ganglion (DRG)
Educational Material
Brochures, White Papers etc.
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Adherent Nucleofection™ of Cryopreserved Rat DRGs
The White Paper shows results for transfecting cultured Lonza cryopreserved rat DRG neurons using the 4D-Nucleofector Y Unit (and adherent Nucleocuvette Strips). -
Cryopreserved Neural Cells and Media
Lonza's Cryopreserved Neurons Offer Comparable Results to Fresh Cells -
Quality and Time: The Real Costs of Your Animal Neural Cell Isolation
Lonza infographic illustrating the benefits of using Lonza's Ready-to-Use Cells -
Primary Cells and Media Reference Guide
A comprehensive collection of Lonza primary cell and media products supporting many research areas. -
Cell List – Integrated Solutions for Primary Cells and Transfection