For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® Y Unit.
Cryopreserved ampule of Human Lung Microvascular Endothelial Cells (HMVEC-L) containing ≥ 500,000 cells
Cryopreserved ampoule of Normal Adult Human Dermal Fibroblasts (NHDF-Ad) containing ≥ 500,000 cells
ProCHO® 4 Protein-free CHO Medium with 0.1% Pluronic® F-68, without L-glutamine, phenol red, hypoxanthine or thymidine, 1 L.
ProCHO® 4 Protein-free CHO Medium with 0.1% Pluronic® F-68, without L-glutamine, phenol red, hypoxanthine or thymidine, 1 L
For adherent electroporation of primary cells, e.g. neuronal or endothelial cells, when working with the 4D-Nucleofector® Y Unit.
Pooled 20-donor male only human hepatocytes that are characterized for multiple CYP450, UGT, SULT, and transporter activities. Ideal for drug metabolite identification, characterization, and turnover kinetics.
Pooled 50-donor, mixed gender human hepatocytes that are characterized for multiple CYP450, UGT, SULT, and transporter activities. Ideal for drug metabolite identification, characterization, and turnover kinetics.
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Troubleshooting Guide - PYROGENT® Gel Clot LAL AssayMost problems encountered in the use of the PYROGENT® Gel Clot Limulus Amebocyte Lysate (LAL) Assay fall into two basic categories - no firm gels are observed in reaction tubes where firm gels are expected or firm gels form in reaction tubes, which are expected to yield negative results. This Tech Tip takes a deeper look into each of these categories and provides troubleshooting guidance.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (English)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (French)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Japanese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Portuguese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Spanish)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Italian)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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Troubleshooting Guide - Gel Clot LAL Assay Procedure (Chinese)A step-by-step guide depicting how to perform a traditional gel clot LAL assay.
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How To Perform The PYROGENT™ Gel Clot LAL AssayThe gel clot LAL assay is a qualitative test that provides simple positive-negative results. This video demonstrates how to perform the assay from start to finish. -
Instructions - PYROGENT™ and PYROGENT™ Plus (Chinese)Detailed instructions for performing the PYROGENT™ and PYROGENT™ Plus Gel Clot LAL Assay