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NHDC - Human Dendritic Cells, 2.5 Million

NHDC - Human Dendritic Cells, 2.5 Million

Catalog #: CC-2701

Cryopreserved ampule of Normal Human Dendritic Cells (NHDC) containing ≥2.5 million cells

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 1’760.00 CHF
EA

Product Overview

Dendritic Cells (DCs) act as the messenger cells of the immune system by processing antigens from pathogens and presenting the antigens to T cells to initiate an immune response. PoieticsTM Normal Human Dendritic Cells are tested for a panel of specific surface markers, including CD11c+, CD86+, CD80+. HLADR+, and CD14- to provide detailed characterization of the cells. These cells can survive up to 7 days in culture with the proper cytokines (IL-4 and GM-CSF) to maintain the cells as immature dendritic cells (iDCs). Cells can be further differentiated to mature dendritic cells with the addition of TNF-α and IL-1β. 

Applications: Antigen presentation and uptake Primary Research Pharmacology Drug-induced immunosuppression of cytokine secretion

Cryopreserved PoieticsTM Normal Human Dendritic Cells (NHDC) are guaranteed to contain ≥3 million viable cells per ampoule. Expression levels are tested for CD80, CD86, HLA-DR, CD11c, and CD14 and provided for information only. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased. Recommended Medium: LGM-3TM Lymphocyte Growth Medium + 50 ng/ml GM-CSF + 50 ng/ml IL-4

Lonza guarantees the performance of CloneticsTM/PoieticsTM cells only if appropriate CloneticsTM/PoieticsTM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

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Benefits

  • All cells test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • Certificate of Analysis provided for every cell lot

 

Storage and Content

1 x Cryopreserved ampule of NHDC containing ≥2.5 million cells

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Instructions - Poietics™ Immune Cell Systems

    Instructions For Proper Use
  • TechSheet - Normal Human Dendritic Cell System

    Technical Information Sheet for Poietics™ Normal Human Dendritic Cell System
  • Instructions for Use - Dendritic Cell (DC) and T Cell Assay from Matched PBMCs

    Protocol for DC and T Cell Assay from Matched PBMCs
  • TechSheet - Poietics™ Human Immune System Cells

    Technical Product Information Sheet
  • Procedure for thawing Poietics™ cells

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Educational Material

Brochures, White Papers etc.

  • Normal Human Dendritic Cells - Antigen Uptake… It’s Easy as APC

    Lonza Application Notes
  • Procedure for thawing Poietics™ cells

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