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eLearning: Working with Gel Clot
Catalog #: LAL-EL-3
This module describes how to work with the gel clot assay, including calculation of the Maximum Valid Dilution (MVD), product validation and the Initial Qualification (IQ) assay.
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eLearning: Troubleshooting the BET
Catalog #: LAL-EL-6
This module explains different types of endotoxin test failures for gel clot and kinetic LAL assays, guides you through possible causes and suggests strategies to help avoid these issues.
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eLearning: Understanding the BET
Catalog #: LAL-EL-2
This introductory module introduces assay mechanisms, the basic assay requirements, the need for endotoxin controls and how Limulus Amebocyte Lysate (LAL) is made.
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eLearning: Overcoming Interference
Catalog #: LAL-EL-5
This module covers causes of interference by stage and type, inhibition vs. enhancement and proposes solutions for the different categories of interfering products.
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eLearning: Introduction to Endotoxin
Catalog #: LAL-EL-1
This module introduces the learner to endotoxin, the effects endotoxin can cause to the body, regulatory compliance and calculating acceptable endotoxin limits.
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eLearning:Working w/ Photometric Methods
Catalog #: LAL-EL-4
This module covers the basic principles of working with photometric methods, includes a demonstration assay video and sections dealing with calculating the MVD, product characterization, product validation and routine testing.
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Instructions - QCL-1000™ Assay (English)Detailed instructions for performing the QCL-1000™ Endpoint Chromogenic LAL Assay
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PYROSTAR™ ES-F Series BrochureThe PYROSTAR™ ES-F series of reagents are endotoxin-specific and unreactive to β-D-Glucan
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QCL-1000™ Endpoint Chromogenic LAL Assay - Technical TipsThe Importance of Glass Tubes /The Problem with PlasticIncubation Method96-well Plates1 EU/ml Standard PreparationVortexing and Mixing Potential Endotoxin Contaminants
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Instructions – Water for Cell CultureData sheet WFI
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Troubleshooting Guide - Testing Oil Solutions with PYROGENT® Gel Clot LAL AssayEndotoxins, due to their lipid character, tend to remain associated with oils and do not readily enter the aqueous phase of water-oil mixtures. This Tech Tip describes the use of a dispersing agent, PYROSPERSE®, to allow for dissociation of the endotoxin from the oils and for subsequent detection of endotoxin in the aqueous phase by the PYROGENT® Gel Clot LAL Assay.
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Troubleshooting Guide - Endpoint Chromogenic LAL Assay Procedure (English)This is a step-by-step guide depicting how to perform the endpoint chromogenic LAL assay in a 96-well plate.
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Instructions - MycoZap™ Mycoplasma Elimination ReagentMycoZap™ Mycoplasma Elimination Reagent Instructions
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Endpoint Chromogenic Limulus Amebocyte Lysate (LAL) Assay Procedure Quick GuideThis is a step-by-step guide depicting how to perform the endpoint chromogenic LAL assay in a 96-well plate. (English)
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Overcoming Assay Inhibition or Enhancement Technical TipsOne of the most time-consuming aspects of endotoxin testing using Limulus Amebocyte Lysate (LAL) is pretreating samples to overcome assay inhibition and enhancement. All assays, independent of methodology, are standardized using endotoxin in water. Lonza offers three solutions for overcoming LAL testing inhibitions
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White-Paper LAL-vs-rFCLonza discusses the transistion From LAL to rFC Assays
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