SF Cell Line 4D-Nucleofector® LV Kit XL (5 x 1 RCT)
SF Cell Line 4D-Nucleofector® LV Kit XL (5 x 1 RCT)
For larger scale transfection of cell lines, e.g. suspension CHO or HEK293, in the LV Nucleocuvette® Cartridge format when working with the 4D-Nucleofector® LV Unit
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Product Overview
The SF Cell Line 4D-Nucleofector® LV Kit XL* is suited for larger scale transfection of cell lines in the LV Nucleocuvette® Cartridge format when working with the 4D-Nucleofector® LV Unit. With the LV Nucleocuvette® Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.
This kit has been determined to be optimal for transfecting following cell lines and many others that use Solution SF as optimal Nucleofector® Solution:
- HEK293 suspension clones
- CHO-S suspension clones
- K-562
- U-937
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
*Note: From the three cell line solutions (SE, SF, SG) that come with the 4D-Nucleofector® System only SF is available for the large-scale formats so far. Solution SF is suited for the vast majority of cell lines that might be used in larger scale applications (e.g. suspension CHO or 293 cells). For other solutions please contact our Scientific Support.
Request More InfoBenefits
- Closed Nucleofection of up to 109 cell lines
- Conditions transferable from existing smaller scale protocols
- Automatic processing via tubing system
- Transfection of up to 20 mL reaction volume in less than 10 minutes
Applications
- For large scale transfection of up to 1 x 109 cells
- Transient production of potential therapeutic proteins or antibodies for construct screening
- Generation of large numbers of transiently modified cell lines for cell-based assays
Storage and Content
5 x 22.5 mL SF Cell Line Nucleofector® Solution
5 x 5 mL Supplement 1
5 x LV Nucleocuvette® Cartridge
10 x 4D-Nucleofector® LV Reservoir
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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CHO [Suspension] Transfection Protocol - 4D-Nucleofector™ LV Unit
Instructions of use for transfection of suspension CHO cells with the 4D-Nucleofector™ LV Unit -
HEK-293 [Suspension] Transfection Protocol - 4D-Nucleofector™ LV Unit
Instructions of use for transfection of suspension HEK-293 cells with the 4D-Nucleofector™ LV Unit
Lonza GS Xceed™ Cells transfected in small or larger scale show comparable IgG4 titers
For a human IgG4 antibody, similar titers of about 100 µg/mL were achieved in small scale (100 µl) and large scale transfections (1 ml fixed cartridge or 10 mL in flow through LV cartridge) in a 6-day batch culture without any feeds. IgG titers were increased by factor 2.5 when using higher seeding densities (not shown here). Switching to a 14-day fed-batch culture led to titers > 1 mg/mL (not shown here).
