Product Overview

Endothelial cells are the primary barrier cells that line blood vessels and sinusoids of the liver. Human Liver-derived Endothelial Cells (HLECs) are isolated and enriched from liver tissue, then cryopreserved for use in a wide range of research models. A unique subtype of LECs, liver sinusoidal endothelial cells, are defined as a subset of cells found at the interface between the liver sinusoids and hepatocytes.

HLECs derived from whole livers lead to a mixed population of vascular endothelial cells and sinusoidal endothelial cells. HLECs characterized for markers of endothelial cells, including vWF maintain appropriate endothelial morphology over limited serial passaging. Measurement of uptake of acetylated LDL as well as CD32b to indicate percentage of the sinusoidal endothelial cell population. The recommended medium for HLECs is EGM^TM 2 which is optimized for endothelial cell health and growth.