Large-Scale Transfection

Efficient, Closed and Scalable Transfection

Experience the new functional unit for the 4D-Nucleofector^TM System which expands our proven technology to large-scale transfection. The LV Unit allows for closed, scalable transfection of larger cell numbers in the range of 1x10⁷ to 1x10⁹ cells.

Transfection protocols can be established in smaller scale using the X Unit and subsequently transferred to the LV Unit without the need for re-optimization. Transferability has been tested for various cell types, including human T cells, CHO-S, HEK293-S, or K562.

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Benefit from

Closed system: Sterile Nucleofection of up to 10⁹ cells
Real scalability: Optimization in small scale
Established protocols: Benefit from 700+ optimized cell types
Simple handling: Minimal training needs
4D-Nucleofector^TM LogWare: Optional operation via 21CFR part11 compliant software

Applications

Ex-vivo modification of human primary cells for cell therapy applications (e.g. genome editing, generation of CAR-T cells)
Transient production of potential therapeutic proteins or antibodies for construct screening
Generation of large numbers of transiently modified primary cells for cell-based assays

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4D-Nucleofector^® LV Unit

Are you interested in seeing our closed system for large-volume Nucleofection in your lab and how it performs for your cells? One of our transfection specialists can come to your lab and perform a transfection experiment with your cell type of interest.

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Two Large-Scale Transfection Formats Available

Researchers can choose between two different Nucleocuvette^TM Cartridges depending on the cell numbers to transfect and their application.

1 mL Nucleocuvette^TM Cartridge

1 mL filling volume
Up to 1x10⁸ cells
Manual filling via sterile injection port

LV Nucleocuvette^TM Cartridge

Up to 20 mL processing volume (in 1 mL steps)
Up to 1x109 cells
Automatic filling via reservoirs or bags

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Transferability from Small to Large Scale

Comparison of various exemplary cell types transfected with pmaxGFP^TM Vector in small volume (100 µL Nucleocuvette^TM Vessels) or larger volumes (1 mL Nucleocuvette^TM Cartridge or LV Nucleocuvette^TM Cartridge) using the same conditions.Transfection efficiency and cell viability were analyzed 24 hours post Nucleofection. Data represent the mean of various independent experiments.

References:

Dever et al. (2016) CRISPR/Cas9 β-globin gene targeting in human haematopoietic stem cells. Nature 539(7629):384-389 (pubmed)
Mendt et al. (2018) Generation and testing of clinical-grade exosomes for pancreatic cancer. JCI Insight 3(8) (pubmed)

Transfer your transfection application from small to large-scale using a straight forward approach.

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Large-Scale Transfection using the 4D-NucleofectorTM LV Unit

Efficient, Closed and Scalable Transfection

Benefit from

Applications

Demo Request

4D-Nucleofector® LV Unit

Two Large-Scale Transfection Formats Available

1 mL NucleocuvetteTM Cartridge

LV NucleocuvetteTM Cartridge

Transferability from Small to Large Scale

Large-Scale Transfection using the 4D-Nucleofector^TM LV Unit

4D-Nucleofector^® LV Unit

1 mL Nucleocuvette^TM Cartridge

LV Nucleocuvette^TM Cartridge