Lonza's MycoAlert® Mycoplasma Detection Assays
Cell culture contaminations with mycoplasma easily go unnoticed for months
Mycoplasma are amongst the smallest viable bacteria and are invisible under standard microscope examination. However, a mycoplasma contamination of cell cultures can seriously impact the reliability, reproducibility, and consistency of experimental results. It presents a major challenge for research, as well as in the manufacturing of bioproducts.
If you suspect mycoplasma contamination of your cells, or if you want to maintain a mycoplasma-free cell culture lab, then you will need a fast and convenient mycoplasma detection assay such as the MycoAlert® Mycoplasma Detection Assay.
The MycoAlert® Mycoplasma Detection Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes which are found in the vast majority of about 200 mycoplasma species, but are not present in eukaryotic cells. No need to culture indicator cell lines, extract DNA or run PCR reactions – this bioluminescence assay uses excess cell culture supernatant so you can:
- Focus on your research and do mycoplasma testing on the fly
- Read out the results within 20 minutes
- Set up easy routine mycoplasma testing in the lab
MycoAlert® Mycoplasma Detection Kit Tutorial
The video shows the workflow for Mycoplasma detection in cell cultures using the MycoAlert® Mycoplasma Detection KitMycoAlert® Mycoplasma Detection Kits – overview
The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit have the same assay principle and the workflow for both assays is the same. However, they do differ in the amount of light each assay emits and therefore have slightly different application ranges.
MycoAlert® Mycoplasma Detection Kits | MycoAlert® PLUS Mycoplasma Detection Kits |
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LT07-118 – 10 tests | LT07-701 – 10 tests |
Protocol download: MycoAlert® Mycoplasma Detection Kit | Protocol download: MycoAlert® PLUS Mycoplasma Detection Kit |
50% off trial version of MycoAlert® Mycoplasma Detection Kit* (includes negative and positive controls) | 50% off trial version of MycoAlert® PLUS Mycoplasma Detection Kit* (includes negative and positive controls) Request Trial Offer |
Is your luminometer compatible with MycoAlert® Products?
The MycoAlert® Mycoplasma Detection Kit requires a suitable tube or plate luminometer, or multi-functional plate reader capable of detecting luminescence. Download the list of recommended luminometers to determine if your luminometer is compatible with the MycoAlert® Mycoplasma Detection Assay.
Alternatively, check out the Lucetta® 2 Luminometer: This versatile, single tube luminometer includes preprogrammed settings for the MycoAlert® Assay.
* This offer is available in participating countries only. Maximum 1 offer per customer per year and as long as stocks last. Discounts are off list prices only. Void where prohibited. Not valid in all countries. Lonza reserves the right to modify this promotional offer at any time without notice.